LA UDES PUBLICA
Fecha de publicación:
2022-07-01
Tipo:
Article
Número de artículo:
e0270995
Identificación:
SCOPUS_ID:85133680823
eID:
2-s2.0-85133680823
Nombre de la revista:
PLoS ONE
Título del artículo:

Multilocus Sequence Typing helps understand the genetic diversity of Cryptosporidium hominis and Cryptosporidium parvum isolated from Colombian patients

Multilocus Sequence Typing has become a useful tool for the study of the genetic diversity and population structure of different organisms. In this study, a MLST approach with seven loci (CP47, MS5, MS9, MSC6-7, TP14, and gp60) was used to analyze the genetic diversity of Cryptosporidium hominis and Cryptosporidium parvum isolated from 28 Colombian patients. Five Cryptosporidium species were identified: C. hominis, C. parvum, Cryptosporidium felis, Cryptosporidium meleagridis, and Cryptosporidium suis. Unilocus gp60 analysis identified four allelic families for C. hominis (Ia, Ib, Id, and Ie) and two for C. parvum (IIa and IIc). There was polymorphic behavior of all markers evaluated for both C. hominis and C. parvum, particularly with the CP47, MS5, and gp60 markers. Phylogenetic analysis with consensus sequences (CS) of the markers showed a taxonomic agreement with the results obtained with the 18S rRNA and gp60 gene. Additionally, two monophyletic clades that clustered the species C. hominis and C. parvum were detected, with a higher number of subclades within the monophyletic groups compared to those with the gp60 gene. Thirteen MLG were identified for C. hominis and eight for C. parvum. Haplotypic and nucleotide diversity were detected, but only the latter was affected by the gp60 exclusion from the CS analysis. The gene fixation index showed an evolutionary closeness between the C. hominis samples and a less evolutionary closeness and greater sequence divergence in the C. parvum samples. Data obtained in this work support the implementation of MLST analysis in the study of the genetic diversity of Cryptosporidium, considering the more detailed information that it provides, which may explain some genetic events that with an unilocus approach could not be established. This is the first multilocus analysis of the intra-specific variability of Cryptosporidium from humans in South America.

Autor(es) UDES:
Farfan-Garcia A.E.
Otros Autores:
Uran-Velasquez J., Alzate J.F., Gomez-Duarte O.G., Martinez-Rosado L.L., Dominguez-Hernandez D.D., Rojas W., Galvan-Diaz A.L., Garcia-Montoya G.M.
Autor Principal:
Uran-Velasquez J.
Áreas del conocimiento:
Multidisciplinary
Acerca de la revista donde se publicó este artículo:

PLoS ONE

Cuartil Q1
Ranking
5108
Tipo
Journal
eISSN
19326203
Región
Northern America
País
United States
Volumen
17
Cobertura
2006-2022
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